REF, in the presence of a total of 48 individuals, has organized three-day youth gathering (March 26-28, 2022) in Kathmandu. This was first youth national gathering in which a total of 48 persons that include 37 youths representing 14 different communities from 12 districts, and 11 individuals from different 6 conservation organization were present. The objective of this gathering was to enhance leadership capacity of the youths through enhancing knowledge and their networks. This gathering was a common platform for the youths to cross-share and cross-learn their knowledge and experiences on bio-cultural conservation. It was delighted with introductory interaction with representatives of the key national conservation organization working in Nepal that include WWF Nepal, IUCN Nepal, NTNC (National Trust for Nature Conservation), Red Panda Network Nepal, Bird Conservation Nepal, DNPWC (Department of National Park and Wildlife Conservation), and Biodiversity Division of the Ministry of Forest and Environment (MoFE). The youth participants realized the importance of understanding bio-cultural issues and their conservation roles and responsibilities. Followed with the refreshing excursion visits to some places inside the Kathmandu valley, the participating youths also discussed about the importance of the formation of national youth network including its vision, mission, membership, governing structure and action plan. Engagement of participating youths in three-day workshop became a milestone for exploring knowledge network and collaboration for bio-cultural conservation.